課程名稱 |
後生遺傳調控機制新知 Frontiers in Epigenetic Regulatory Mechanisms |
開課學期 |
101-1 |
授課對象 |
生物資源暨農學院 生物科技研究所 |
授課教師 |
林劭品 |
課號 |
Biot8012 |
課程識別碼 |
642ED0140 |
班次 |
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學分 |
2 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
星期一9,A(16:30~19:15) |
上課地點 |
生技所410 |
備註 |
本課程以英語授課。 限博士班 總人數上限:20人 外系人數限制:15人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1011EpiMechanism |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
Depends on the background of the students, around 1/3 of the lectures will be given by the instructor for introducing basic concepts in Epigenetic regulation of gene silencing, including DNA methylation, modification of chromatin packaging proteins and functional non-coding RNAs. These will cover the physiological process including Genomic Imprinting, Sex Chromosome Compensation, Silencing of Endogenous Retrotransposons and Newly Infected Viral Sequences, Stem Cell Biology. In addition, the involvement of Epigenetic Regulatory Mechanisms in Carcinogenesis, Animal Cloning, Reprogramming and Regenerative Medicine will be discussed. For the rest of the classes, students will be assigned the most updated original research papers and conference articles as well as the milestone publications. They will be randomly divided into 2 groups for each assignment. One group will be presenting the articles and students in the other group are expected to challenge the papers from the experimental design, the methodology to the interpretation of the data. Students in the 1st group should defend for the papers as if it’s their manuscript. Students from the 2nd group should also provide constructive advice as how to improve the manuscript so that it can be published in a higher impact journal. |
課程目標 |
On top of learning the basic concepts of Epigenetic Regulatory Mechanisms, keeping abreast of the most updated research internationally, students are expected to develop the ability to critically evaluate and interpret published research articles. In addition, they will learn the art of reviewing as well as defending a manuscript for publication. |
課程要求 |
Preferably having studied one of the following subjects: (Introductory) Epigenetics, Special Topics in Reprogramming, Genetics and Epigenetics in Germ and Stem Cells |
預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
Updated journal articles and reviews; “Epigenetics: Concepts, Theories, Paradigms and Mechanisms” and “Stem cell and Regenerative Medicine” CD-Roms (A Henry StewartTalks Series in the Life Sciences), National and International Conference Abstracts and Papers |
評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
Week 1 |
9/10 |
The appliction og NGS in Epigenetic studies
Cis-regulatory control of corticospinal system development and evolution.
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Week 2 |
9/17 |
Efficient genetic midification and germ-line transmission of primordial germ cells usung piggyBac and Tol2 transposons.pdf |
Week 3 |
9/24 |
A genome-wide RNAi screen reveals determinants of human embryonic stem
cell identity.
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Week 4 |
10/01 |
Differential genomic imprinting and expression of imprinted microRNAs in testes-derived male germ-line stem cells in mouse |
Week 5 |
10/08 |
Function, Targets, and Evolution of Caenorhabditis elegans piRNAs |
Week 6 |
10/15 |
Functions of PIWI proteins in spermatogenesis
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Week 7 |
10/22 |
NEUROG3 Is a Critical Downstream Effector for STAT3-Regulated Differentiation of Mammalian Stem and Progenitor Spermatogonia |
Week 8 |
10/29 |
The Basement Membrane of Hair Follicle Stem Cells Is a Muscle Cell Niche |
Week 9 |
11/05 |
In Vivo Control of CpG and Non-CpG DNA Methylation by DNA Methyltransferases.
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Week 10 |
11/12 |
Gender-Specific Hierarchy in Nuage Localization of PIWI-Interacting RNA Factors in Drosophila
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Week 11 |
11/19 |
Heritable genome-wide variation of gene
expression and promoter methylation between
wild and domesticated chickens |
Week 12 |
11/26 |
Deep-Sequencing Reveals Specific microRNAs in An Autoregressive Tumor Model |
Week 13 |
12/03 |
Gene Expression and DNA Methylation Status of Chicken
Primordial Germ Cells |
Week 16 |
12/24 |
piRNAs Initiate an Epigenetic Memory of Nonself RNA in the C. elegans |
Week 17 |
12/31 |
The PIWI protein acts as a predictive marker for human gastric cancer
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